What question did this study set out to answer?

Characterize the structural proteins comprising bacteriophage M13 particles and determine the major phage-encoded proteins synthesized during host cell infection.

March 1, 1969Proceedings of the National Academy of SciencesOpen Access

The Proteins of Bacteriophage M13

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Authors

THTimothy J. HenryUniversity of Wisconsin System DPDavid PrattThe University of Texas Southwestern Medical Center

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Implication

Biochemical analysis reveals the structural and replication proteins of bacteriophage M13 in infected bacterial cells, indicating that parental double-stranded DNA serves as the primary...

Key Points

Characterize the structural proteins comprising bacteriophage M13 particles and determine the major phage-encoded proteins synthesized during host cell infection.
Analyzed purified filamentous coliphage M13 virions to determine the composition and molecular weights of structural coat proteins.
Measured intracellular phage-specific protein expression in host cells infected with wild-type M13 and gene 2 replication-defective mutants.
Identified the gene 8 major coat protein and a single-copy gene 3 minor coat protein (~70,000 daltons) responsible for host cell attachment.
Determined that the gene 5 product (~8,000 daltons) is the principal intracellular protein synthesized and directs single-stranded DNA production.
Observed near-normal rates of phage protein synthesis when double-stranded DNA replication was blocked by gene 2 mutations, indicating the initial duplex DNA serves as the primary transcription template.

Cite This Study

Henry et al. (1969) studied this question.

synapsesocial.com/papers/6a879baca4fe135464e9cab5 https://doi.org/10.1073/pnas.62.3.800

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Implication

Biochemical analysis reveals the structural and replication proteins of bacteriophage M13 in infected bacterial cells, indicating that parental double-stranded DNA serves as the primary...

The Proteins of Bacteriophage M13

Discussion

Member takes

Implication

Key Points

Cite This Study

The Proteins of Bacteriophage M13

Discussion

Member takes

Implication

Key Points

Cite This Study

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