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August 1, 1991Journal of BacteriologyOpen Access

Identification of the promoter region of the ribosome-releasing factor cistron (frr)

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Population

Escherichia coli (in vivo and in vitro models for RRF promoter analysis)

Comparison

Deletion analysis of the 5' proximal sequences… vs lac promoter (for strength comparison)

Design

Preclinical

Authors

ISIchiro ShimizuTokyo University of ScienceAKAkira KajiUniversity of Pennsylvania

Discussion

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Implication

No immediate clinical relevance; leaves open the regulatory role of this promoter in bacterial translation.

Structured PICO

P
Population
Escherichia coli (in vivo and in vitro models for RRF promoter analysis)
I
Intervention
Deletion analysis of the 5' proximal sequences of frr fused to the chloramphenicol acetyltransferase reporter gene, and gel retardation analysis with E. coli RNA polymerase
C
Comparator
lac promoter (for strength comparison)
O
Outcome
Identification and characterization of the RRF promoter regionsurrogate

The study identifies and characterizes the promoter region of the ribosome-releasing factor cistron in E. coli, demonstrating its strength is comparable to the lac promoter but independent of cAMP regulation.

Cite This Study

Shimizu et al. (1991) studied this question.

synapsesocial.com/papers/6a87a323a9eb41bcdfc4d6c3https://doi.org/10.1128/jb.173.16.5181-5187.1991
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