What question did this study set out to answer?

To develop an ultrasensitive detection platform using europium(III) nanoparticles and time-resolved fluorometry capable of resolving individual prostate-specific antigen molecules in microtiter plates.

March 1, 2001Clinical Chemistry

Europium Nanoparticles and Time-resolved Fluorescence for Ultrasensitive Detection of Prostate-specific Antigen

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Authors

HHHarri HärmäUniversity of Turku TSTero SoukkaUniversity of Turku TLTimo LövgrenQueen's University Belfast

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Implication

In vitro assay demonstrates zeptomole-level detection of prostate-specific antigen using europium nanoparticles, indicating broad potential for ultrasensitive clinical diagnostics.

Key Points

To develop an ultrasensitive detection platform using europium(III) nanoparticles and time-resolved fluorometry capable of resolving individual prostate-specific antigen molecules in microtiter plates.
Coated 107-nm nanoparticles carrying >30,000 β-diketone-entrapped europium molecules with streptavidin.
Applied the nanoparticles to trace biotinylated prostate-specific antigen (PSA) in microtiter wells using time-resolved fluorometry and a time-resolved fluorescence microscope with a 10× objective.
Achieved a lower detection limit for biotinylated PSA of 0.38 ng/L, equivalent to 10 fmol/L or 60 zeptomoles (60 × 10⁻²¹ moles).
Successfully visualized single nanoparticles representing individual PSA molecules in microtiter plate wells and detected single nanoparticles in solution via standard time-resolved plate fluorometry.

Cite This Study

Härmä et al. (2001) studied this question.

synapsesocial.com/papers/6a87aa865f0f8a636faca26d https://doi.org/10.1093/clinchem/47.3.561

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Implication

In vitro assay demonstrates zeptomole-level detection of prostate-specific antigen using europium nanoparticles, indicating broad potential for ultrasensitive clinical diagnostics.

Europium Nanoparticles and Time-resolved Fluorescence for Ultrasensitive Detection of Prostate-specific Antigen

Discussion

Member takes

Implication

Key Points

Cite This Study

Europium Nanoparticles and Time-resolved Fluorescence for Ultrasensitive Detection of Prostate-specific Antigen

Discussion

Member takes

Implication

Key Points

Cite This Study

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