Identification and characterization of voltage-sensitive calcium channels in neuronal clonal cell lines
Population
Three neuronal clonal cell lines: neuroblastoma X Chinese hamster brain hybrid, neuroblastoma X glioma…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Hypothesis-generating for neuronal calcium channel pharmacology; leaves open translation to cardiovascular therapies.
Structured PICO
P
Population
Three neuronal clonal cell lines: neuroblastoma X Chinese hamster brain hybrid (NCB-20), neuroblastoma X glioma hybrid (NG108-15), and neuroblastoma (N4TG1)
I
Intervention
Depolarization with 50 mM KCl or 50 microM veratridine; treatment with organic calcium antagonists (nitrendipine, cinnarizine, verapamil, diltiazem); cellular differentiation agents
O
Outcome
Net 45Ca2+ uptakesurrogate
Voltage-sensitive calcium channels in neuronal clonal cell lines can be altered by cellular differentiation and are blocked by organic calcium channel antagonists at very low concentrations.
Cite This Study
Freedman et al. (1984) studied this question.