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Comparative biochemical analysis reveals structural, kinetic, and immunological identity between rat heart and liver citrate synthases, indicating a single enzyme form across both tissues.
The citrate synthases from rat heart and rat liver have been purified. These homogeneous proteins have a molecular weight of about 1 x 105 and are composed of 2 apparently identical subunits. Kinetic constants for acetyl coenzyme A and oxaloacetate and the Ki for ATP are the same for both enzymes. Immunological studies using antibody to the rat heart enzyme also indicate that the enzymes are identical.
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Moriyama et al. (1971) studied this question.
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