Kinetics of hydrolase production by microorganisms

The cnzyme-forming systcms (EFSs) concerned with the amylase of Bacillus subtilis.and the glucamylase and acid protease of Aspergillus niger, have been shown to be highly stable in the cultural conditions applied owing to the low decay rate of mRNAs specific for these respective enzymes.The quantity per cell of mRNAs for any of thcse enzymes is rcgardcd as limiting the specific rate of cnzymc production in the conditions used for enzyme production.Investigations on the repression, dercpression and the prcferential synthesis of thcse hydrolases have led to simple hypothctical relationships.r to X a;ß;y;b 8= e-value at time t 0 (units x mg-1 h-1 ): = t:-value at time tm (units x mg-1 h-1 ): = rate constant for the decay of cellular RNA in nongrowing phase(h-1 ): = specific growth rate (h-1 ): = averag:e .u-value in the time interval (h ~ 1 ).