Safety evaluation of the food enzyme protein‐glutamine glutaminase from the non‐genetically modified Chryseobacterium proteolyticum strain AE‐PG

strain AE-PG by Amano Enzyme Inc. The food enzyme was considered free from viable cells and DNA of the production organism. It is intended to be used in three food manufacturing processes. Dietary exposure was estimated to be up to 0.089 mg total organic solids (TOS)/kg body weight (bw) per day in European populations. Genotoxicity tests did not indicate a safety concern. The systemic toxicity was assessed by means of a repeated dose 90-day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 93 mg TOS/kg bw per day, the highest dose tested, which when compared with the estimated dietary exposure, resulted in a margin of exposure of at least 1045. A search for the homology of the amino acid sequence of the protein-glutamine glutaminase to known allergens was made and no match was found. The Panel considered that a risk of allergic reactions upon dietary exposure cannot be excluded, but that the likelihood is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns, under the intended conditions of use.