What question did this study set out to answer?

To evaluate whether primary muscle cell culture transplantation can successfully restore the laminin alpha2 chain in the basal lamina of laminin-deficient dystrophic mice.

April 1, 1996The Journal of Cell BiologyOpen Access

Partial laminin alpha2 chain restoration in alpha2 chain-deficient dy/dy mouse by primary muscle cell culture transplantation.

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To evaluate whether primary muscle cell culture transplantation can successfully restore the laminin alpha2 chain in the basal lamina of laminin-deficient dystrophic mice.
Transplanted allogeneic primary muscle cell cultures expressing a beta-galactosidase transgene or histocompatible primary muscle cell cultures into muscles of dy/dy mice.
Administered FK506 immunosuppression in noncompatible allogeneic models and compared primary cell outcomes to conditionally immortalized pure myogenic cells in vitro and in vivo.
Partial laminin alpha2 chain restoration occurred in all immunologically controlled dy/dy mice, with restoration levels depending on recipient age, muscle pretreatment, and post-transplantation duration.
Laminin alpha2 deposition radiated segmentally from the injection site and did not strictly colocalize with donor-derived muscle fibers, whereas conditionally immortalized pure myogenic cells formed fibers but failed to express laminin alpha2 in vivo.

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Implication

Animal study demonstrates partial laminin alpha2 restoration in dystrophic mice via primary muscle cell transplantation, suggesting not all myogenic cells effectively deposit the protein.

Cite This Study

Vilquin et al. (1996) studied this question.

synapsesocial.com/papers/6a87c52b6e1dca10b7bb58b4 https://doi.org/10.1083/jcb.133.1.185

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Implication

Animal study demonstrates partial laminin alpha2 restoration in dystrophic mice via primary muscle cell transplantation, suggesting not all myogenic cells effectively deposit the protein.

Partial laminin alpha2 chain restoration in alpha2 chain-deficient dy/dy mouse by primary muscle cell culture transplantation.

Key Points

Discussion

Implication

Cite This Study

Partial laminin alpha2 chain restoration in alpha2 chain-deficient dy/dy mouse by primary muscle cell culture transplantation.

Key Points

Discussion

Implication

Cite This Study

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