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January 1, 1974International Journal of Systematic Bacteriology

Observations on Anaerobic Glucose Utilization Tests in Staphylococcus-Micrococcus Identification

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Authors

GDG. H. G. DavisBoston UniversityBHB. HOYLINGThe University of Queensland

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Implication

Comparative laboratory evaluation finds superior reliability for pH-based glucose tests over thioglycolate deep-tube assays in gram-positive cocci, suggesting pH testing yields clearer classification.

Key Points

  • To evaluate the diagnostic reliability of Hugh and Leifson anaerobic acid production tests compared to thioglycolate deep-tube growth tests for distinguishing Staphylococcus and Micrococcus isolates.
  • Assayed 212 freshly isolated and reference cultures of gram-positive cocci using Hugh and Leifson glucose oxidation-fermentation tests and thioglycolate deep-tube growth tests.
  • Tested 106 strains designated as Baird-Parker micrococcal subgroups M1 through M4 to examine specific subgroup discrepancies.
  • Assessed modified variations of pH-indicator tests and anaerobic growth detection protocols to evaluate inter-observer consistency and diagnostic sensitivity.
  • Anaerobic glucose metabolism was detected in 85 of 212 cultures via the Hugh and Leifson acid-production test compared to 138 of 212 cultures in the thioglycolate growth test.
  • Among 106 Baird-Parker subgroup M1 through M4 micrococci, 55 displayed anaerobic growth in thioglycolate deep tubes, demonstrating that thioglycolate growth failed to clarify subgroup differentiation.
  • Modified growth detection assays exhibited high observer variability, whereas pH-indicator tests demonstrated equivalent diagnostic sensitivity alongside superior interpretive reproducibility.

Cite This Study

Davis et al. (1974) studied this question.

synapsesocial.com/papers/6a87c7519842aaac2ffb0df2https://doi.org/10.1099/00207713-24-1-1
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