Pancreatic extracts in relation to lactic acid formation in muscle

THE pancreatic factor of Winfield and Hopkins [1915] which inhibits the formation of lactic acid in muscle and muscle extracts, has been shown The first portion of the work herein reported was carried out, using a new technique to be described, with a view to discovering the properties of the pancreatic factor and its distribution throughout various body tissues. Results were soon obtained, however, which led us to suspect the conclusions of former workers in regard to the possible identity of this factor with other tissue constituents. It seems to have been established that neither trypsin nor insulin is responsible for the inhibition; and Downes [1927], Reay [1927] and Ronzoni [1927] state that in their opinion the inhibitory effect of pancreas and pancreatic extracts is not due to amylase. Our preliminary results, however, indicated the necessity of further work in this connection.