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Stability is a crucial factor influencing applicability of industrial biocatalysts, and enzymatic operations in partial organic solvents and higher temperatures are preferable since organic solvents may improve the solubility of the substrates, and the reaction rates are often higher at elevated temperatures. This review aims to summarize recent advances for engineering enzymes to meet the industrial needs for solvent and thermostability and offer insights in the advances in methodologies utilizing B-factors, ancestral reconstructions, or machine learning approaches.
Yuan et al. (Wed,) studied this question.