November 15, 1991Proceedings of the National Academy of SciencesOpen Access

Poliovirus thiol proteinase 3C can utilize a serine nucleophile within the putative catalytic triad.

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Population

Poliovirus 3C proteinase

Design

Preclinical

Authors

MLMark A. LawsonUniversity of California, San Diego BSBert L. SemlerUniversity of California, Irvine

Discussion

Member takes

Implication

Extends viral protease catalytic diversity models; leaves open whether 3C enzymes represent serine protease ancestors.

Structured PICO

Population

Poliovirus 3C proteinase

Intervention

Mutations at putative active site and non-active site residues, specifically substituting a serine at position 147

Outcome

Effect on 3C-mediated protein processingsurrogate

Picornavirus 3C proteinases can utilize a serine nucleophile, suggesting they may represent an ancestral enzyme class to highly divergent serine proteases.

Cite This Study

Lawson et al. (1991) studied this question.

synapsesocial.com/papers/6a87cc046e1dca10b7bb5b6a https://doi.org/10.1073/pnas.88.22.9919

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Poliovirus thiol proteinase 3C can utilize a serine nucleophile within the putative catalytic triad.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Poliovirus thiol proteinase 3C can utilize a serine nucleophile within the putative catalytic triad.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider