Key Points
- To determine the high-resolution three-dimensional crystal structure of coxsackievirus B3.
- Crystallized icosahedral CVB3 virions in monoclinic space group P2(1) with two virions per asymmetric unit, establishing 120-fold non-crystallographic redundancy.
- Collected X-ray diffraction data extending to 2.7 Å resolution with 55% completeness to 3.0 Å.
- Determined particle orientations via 3.0–3.2 Å self-rotation functions and carried out phase refinement with real-space electron-density averaging using human rhinovirus 14 (HRV14) as an initial model.
- Identified deviations from pseudo-R32 symmetry, successfully resolving particle orientations and positions among six ambiguous monoclinic space-group settings.
- Constructed and refined the initial structural model of the CVB3 virion to 3.5 Å resolution using X-PLOR.
Structured PICO
PPopulationCoxsackievirus B3 (CVB3) particles
IInterventionX-ray crystallography and structure determination
OOutcomeCrystal structure of CVB3 to 3.5 Å resolution
The determination of the CVB3 crystal structure to 3.5 Å resolution provides foundational structural biology insights into this virus.