November 1, 1997Journal of VirologyOpen Access

Synchronous replication of poliovirus RNA: initiation of negative-strand RNA synthesis requires the guanidine-inhibited activity of protein 2C

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Population

HeLa S10 extracts translating poliovirion RNA

Comparison

Guanidine HCl (2 mM) addition and removal vs Reactions lacking guanidine or different timing…

Design

Preclinical

Authors

Discussion

Clarifies 2C as poliovirus replication target; leaves open translation to human antivirals.

Population

HeLa S10 extracts translating poliovirion RNA

Intervention

Guanidine HCl (2 mM) addition and removal

Comparator

Reactions lacking guanidine or different timing of guanidine addition

Outcome

Initiation and elongation of negative-strand and positive-strand RNA synthesissurrogate

Protein 2C is required specifically for the initiation of negative-strand RNA synthesis in poliovirus replication.

Barton et al. (1997) studied this question.

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