September 1, 1994The Journal of Cell BiologyOpen Access

Antibodies probe for folded monomeric myosin in relaxed and contracted smooth muscle.

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Population

Avian gizzard cryosections (smooth muscle cells)

Comparison

Labeling with fluorescently conjugated… vs Comparison between relaxed and contracted…

Design

Preclinical

Authors

AHArie HorowitzThe University of Texas at Austin KTK M TrybusUniversity of Vermont DBDeanna BowmanUniversity of Akron

Discussion

Member takes

Implication

Challenges myosin filament dynamics as regulator in smooth muscle; hypothesis-generating for human contractile mechanisms.

Structured PICO

Population

Avian gizzard cryosections (smooth muscle cells)

Intervention

Labeling with fluorescently conjugated monoclonal antibody specific for folded monomeric conformation or antibody against the tip of the tail

Comparator

Comparison between relaxed and contracted states, and alpha-toxin permeabilized gizzard in solvent

Outcome

Fluorescence intensity of folded monomeric myosinsurrogate

The study demonstrates that myosin assembly/disassembly is unlikely to play a major role in regulating the contraction/relaxation cycle in smooth muscle cells.

Cite This Study

Horowitz et al. (1994) studied this question.

synapsesocial.com/papers/6a87d369f8efd6d7e4fb17fe https://doi.org/10.1083/jcb.126.5.1195

View Full Paper

Ask AI

Bookmark

Antibodies probe for folded monomeric myosin in relaxed and contracted smooth muscle.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Antibodies probe for folded monomeric myosin in relaxed and contracted smooth muscle.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider