Effect of temperature and polymer type on gel permeation chromatography

A series of polyisobutene and polystyrene fractions was subjected to gel‐permeation chromatography at 150°C. The two types resulted in distinctly different calibration curves in a plot of projected, extended chain length versus elution volume. The average end‐to‐end distances of the samples were determined by intrinsic viscosity measurements. It was found that these data plotted versus elution volume could be represented by a common curve for both polymer types. The elution volumes of the polyisobutene series were determined at three additional temperatures, 35, 70, and 110°C. It could be shown that elution volume is again determined by polymer coil size at the temperature of measurement.