What question did this study set out to answer?

To evaluate whether a two-color interphase fluorescence in situ hybridization assay can reliably detect 11q13 breakpoints to distinguish mantle cell lymphoma from other B-cell lymphomas.

August 1, 2000American Journal of Clinical Pathology

Detection of Chromosome 11q13 Breakpoints by Interphase Fluorescence In Situ Hybridization

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Authors

RKRuth L. KatzAstraZeneca (United Kingdom)NCNancy P. CarawayThe University of Texas MD Anderson Cancer Center JGJun GuHebei North University

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Implication

Diagnostic validation study reveals elevated 11q13 signals in mantle cell lymphoma, indicating interphase FISH provides rapid diagnostic utility.

Key Points

To evaluate whether a two-color interphase fluorescence in situ hybridization assay can reliably detect 11q13 breakpoints to distinguish mantle cell lymphoma from other B-cell lymphomas.
Assessed cytologic specimens from 11 mantle cell lymphomas (MCLs) and 32 other B-cell non-Hodgkin lymphomas (NHLs) (total N=43).
Applied a two-color FISH assay pairing an 11q13 probe centered on CCND1 with a centromeric probe (CEP11), scoring nuclei across 200 cells per case to calculate the 11q13/CEP11 ratio.
All 11 MCL cases showed three or more 11q13 signals in a mean of 74.8% of nuclei (range 57%–90%) and an elevated mean 11q13/CEP11 ratio of 1.38.
In other B-cell NHLs, three or more 11q13 signals appeared in a mean of 9.2% of nuclei with a mean 11q13/CEP11 ratio of 0.99.
Elevated 11q13/CEP11 ratios occurred in two non-MCL cases (1.15 and 1.30), with conventional cytogenetics confirming a t(5;11)(q31;q13) in the former.

Cite This Study

Katz et al. (2000) studied this question.

synapsesocial.com/papers/6a87edac514a20627548fb97 https://doi.org/10.1309/69ej-rfm5-e976-butp

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Implication

Diagnostic validation study reveals elevated 11q13 signals in mantle cell lymphoma, indicating interphase FISH provides rapid diagnostic utility.

Detection of Chromosome 11q13 Breakpoints by Interphase Fluorescence In Situ Hybridization

Discussion

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Implication

Key Points

Cite This Study

Detection of Chromosome 11q13 Breakpoints by Interphase Fluorescence In Situ Hybridization

Discussion

Member takes

Implication

Key Points

Cite This Study

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