What question did this study set out to answer?

To determine whether murine and human anti-lipid A IgM monoclonal antibodies effectively bind bacterial lipopolysaccharide and neutralize its endotoxic activity.

January 1, 1993The Journal of Experimental MedicineOpen Access

Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide.

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Authors

HWH. Shaw WarrenHarvard University SAS F AmatoIstituto di Fisiologia Clinica CFCatherine FittingInstitut Pasteur

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Implication

In vitro study reveals poor binding and failure of anti-lipid A monoclonal antibodies to neutralize lipopolysaccharide, highlighting doubts about their efficacy in gram-negative sepsis.

Key Points

To determine whether murine and human anti-lipid A IgM monoclonal antibodies effectively bind bacterial lipopolysaccharide and neutralize its endotoxic activity.
Measured binding between two IgM anti-lipid A monoclonal antibodies and tritiated lipopolysaccharide from multiple gram-negative strains using a fluid-phase radioimmunoassay.
Assessed in vitro neutralization of lipopolysaccharide across Limulus amoebocyte lysate assays, murine splenocyte mitogenic assays, and cytokine release assays (IL-1, IL-6, and TNF) in cultured human monocytes and whole blood.
Both monoclonal antibodies displayed modest binding to lipopolysaccharide that was only slightly higher than control preparations, requiring large antibody excesses to detect.
Neither antibody neutralized lipopolysaccharide biological activity across Limulus lysate, splenocyte mitogenesis, or human monocyte cytokine production assays, despite the presence of large molar excesses.

Cite This Study

Warren et al. (1993) studied this question.

synapsesocial.com/papers/6a87fc50eb5451a844aa654d https://doi.org/10.1084/jem.177.1.89

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Implication

In vitro study reveals poor binding and failure of anti-lipid A monoclonal antibodies to neutralize lipopolysaccharide, highlighting doubts about their efficacy in gram-negative sepsis.

Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide.

Discussion

Member takes

Implication

Key Points

Cite This Study

Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide.

Discussion

Member takes

Implication

Key Points

Cite This Study

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