May 3, 2007Journal of Biological ChemistryOpen Access

Silencing of Insulin-like Growth Factor-binding Protein-2 in Human Glioblastoma Cells Reduces Both Invasiveness and Expression of Progression-associated Gene CD24

View Full Paper

Ask AI

Bookmark

Population

Human glioblastoma multiforme (GBM) cell lines (U251, YKG-1, A172) and nude mice

Comparison

Silencing of IGFBP-2 using short hairpin-RNA… vs Control cells (implied)

Design

Preclinical

Authors

TFTsuyoshi FukushimaHarvard University TTTomoaki Tezuka TSTakeshi ShimomuraTokyo University of Agriculture and Technology

Discussion

Member takes

Implication

Supports IGFBP-2/CD24 as glioblastoma targets; hypothesis-generating from Level 5 data and requires clinical validation.

Structured PICO

Population

Human glioblastoma multiforme (GBM) cell lines (U251, YKG-1, A172) and nude mice

Intervention

Silencing of IGFBP-2 using short hairpin-RNA (shRNA) expression retroviral vectors; inactivation of CD24; forced overexpression of CD24; transfection with an IGFBP-2 expression plasmid

Comparator

Control cells (implied)

Outcome

Cellular invasiveness, saturation density in vitro, and tumorigenicity in nude micesurrogate

In human glioblastoma cells, IGFBP-2 enhances cellular invasiveness and tumorigenicity, at least in part by modulating the expression of the progression-associated gene CD24.

Cite This Study

Fukushima et al. (2007) studied this question.

synapsesocial.com/papers/6a87fe2ec0c64b7dddeb3344 https://doi.org/10.1074/jbc.m609567200

View Full Paper

Ask AI

Bookmark

Silencing of Insulin-like Growth Factor-binding Protein-2 in Human Glioblastoma Cells Reduces Both Invasiveness and Expression of Progression-associated Gene CD24

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Silencing of Insulin-like Growth Factor-binding Protein-2 in Human Glioblastoma Cells Reduces Both Invasiveness and Expression of Progression-associated Gene CD24

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider