What question did this study set out to answer?

To determine whether cationic antibacterial proteins in polymorphonuclear leukocytes are localized to a distinct class of subcellular granules separable from classic enzyme-rich granules.

March 7, 1969Science

Cationic Protein-Bearing Granules of Polymorphonuclear Leukocytes: Separation from Enzyme-Rich Granules

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To determine whether cationic antibacterial proteins in polymorphonuclear leukocytes are localized to a distinct class of subcellular granules separable from classic enzyme-rich granules.
Fractionated subcellular particles of polymorphonuclear leukocytes using zonal density gradient centrifugation.
Assayed fractions for cationic antibacterial proteins alongside enzyme markers, including acid phosphatase, alkaline phosphatase, and lysozyme.
Profiled and compared granule contents across polymorphonuclear leukocytes, normal macrophages, BCG-stimulated macrophages, and liver cells.
Cationic antibacterial proteins sedimented within a distinct subcellular particle completely separable from granules containing acid phosphatase, alkaline phosphatase, and lysozyme.
Normal macrophages, BCG-stimulated macrophages, and liver cells lacked the cationic protein-bearing particle and its associated proteins, despite sharing slower-sedimenting enzyme-rich particles.

Authors

HZH. I. ZeyaUniversity of North Carolina at Chapel Hill JSJohn K. SpitznagelUniversity of Maryland, Baltimore

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Implication

Experimental study demonstrates distinct cationic antibacterial protein granules in polymorphonuclear leukocytes, indicating specialized antimicrobial compartments separate from enzyme-rich lysosomes.

Cite This Study

Zeya et al. (1969) studied this question.

synapsesocial.com/papers/6a87fe75eb5451a844aa673a https://doi.org/10.1126/science.163.3871.1069

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Cationic Protein-Bearing Granules of Polymorphonuclear Leukocytes: Separation from Enzyme-Rich Granules

Key Points

Discussion

Implication

Cite This Study

Cationic Protein-Bearing Granules of Polymorphonuclear Leukocytes: Separation from Enzyme-Rich Granules

Key Points

Discussion

Implication

Cite This Study

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