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March 1, 2003Journal of Clinical MicrobiologyOpen Access

Construction and Evaluation of Internal Control DNA for PCR Amplification of Chlamydia trachomatis DNA from Urine Samples

Katy Beaumont Jean Marie Sueur J Orfila

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Abstract

An internal control DNA (ICD) with the same primer binding sequences as the target Chlamydia trachomatis DNA was constructed and evaluated in a PCR assay with immunoenzymatic detection. One hundred urine specimens were tested, and 23 were found to contain inhibitors of the PCR, if not subjected to DNA extraction prior to amplification. Coamplification and detection of the ICD appeared to be a useful method for estimating the effects of inhibitors on C. trachomatis DNA amplification.

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Beaumont et al. (2003) studied this question.

synapsesocial.com/papers/6a88025caf2c2bd0114de85c https://doi.org/10.1128/jcm.41.3.1274-1276.2003

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