Stimulation of gene expression in neonatal rat ventricular myocytes by Ras is mediated by Ral guanine nucleotide dissociation stimulator (Ral.GDS) and phosphatidylinositol 3-kinase in addition to Raf
Population
Cultured neonatal rat ventricular myocytes
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Ras pathway data from neonatal myocytes should not change practice; leaves open effector roles in human cardiac hypertrophy.
Structured PICO
P
Population
Cultured neonatal rat ventricular myocytes
I
Intervention
Transfection with oncogenic (V12) Ras mutants (V12HRas, E37G, D38E, Y40C) and constitutively active PI-3-K mutant (p110K227E)
O
Outcome
Gene expression (ANF-LUX, SkM-alpha-actin-LUX, c-fos-serum-response element-LUX) and cell sizesurrogate
Raf, Ral.GDS, and PI-3-K can transduce transcriptional responses to V12HRas in neonatal ventricular myocytes, but specific induction of hypertrophic genes is not mediated through PI-3-K.
Cite This Study
Fuller et al. (1998) studied this question.