What question did this study set out to answer?

To evaluate and compare the effects of insulin and phorbol esters on the subcellular redistribution of individual protein kinase C (PKC) isoforms in rat adipocytes.

November 15, 1992Biochemical JournalOpen Access

Effects of insulin and phorbol esters on subcellular distribution of protein kinase C isoforms in rat adipocytes

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Authors

RFRobert V. FareseUniversity of South Florida MSMary L. StandaertJoslin Diabetes Center AFAlan FrançoisCentre de Recherche en Mathématiques de la Décision

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Implication

Laboratory study reveals distinct translocation patterns of protein kinase C isoforms in rat adipocytes, indicating stimulus-specific regulation of cellular signaling pathways.

Key Points

To evaluate and compare the effects of insulin and phorbol esters on the subcellular redistribution of individual protein kinase C (PKC) isoforms in rat adipocytes.
Treated isolated rat adipocytes with insulin or phorbol ester agonists.
Separated cells into cytosolic and membrane fractions to evaluate immunoreactive levels of PKC-alpha, PKC-beta, PKC-gamma, PKC-delta, PKC-epsilon, and PKC-zeta.
Both agonists rapidly decreased cytosolic and/or increased membrane levels of PKC-alpha, PKC-beta, PKC-gamma, and PKC-epsilon, though phorbol esters induced a markedly greater plasma membrane translocation of PKC-alpha than insulin.
Insulin selectively triggered the translocation of PKC-beta to the plasma membrane and altered PKC-zeta distribution, whereas phorbol esters had no effect on these isoforms.
Neither agonist altered the subcellular localization of PKC-delta, which was detected exclusively in the membrane fraction.

Cite This Study

Farese et al. (1992) studied this question.

synapsesocial.com/papers/6a8811454c82be800eeb3ff6 https://doi.org/10.1042/bj2880319

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Implication

Laboratory study reveals distinct translocation patterns of protein kinase C isoforms in rat adipocytes, indicating stimulus-specific regulation of cellular signaling pathways.

Effects of insulin and phorbol esters on subcellular distribution of protein kinase C isoforms in rat adipocytes

Discussion

Member takes

Implication

Key Points

Cite This Study

Effects of insulin and phorbol esters on subcellular distribution of protein kinase C isoforms in rat adipocytes

Discussion

Member takes

Implication

Key Points

Cite This Study

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