What question did this study set out to answer?

To determine the compartmental kinetics and sequence motifs governing the intracellular trafficking and surface display of GLUT1 and GLUT4 in fibroblasts and adipocytes.

November 1, 1995Biochemistry

Kinetic analysis of glucose transporter trafficking in fibroblasts and adipocytes

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To determine the compartmental kinetics and sequence motifs governing the intracellular trafficking and surface display of GLUT1 and GLUT4 in fibroblasts and adipocytes.
Constructed mathematical compartmental models to analyze the transport kinetics of overexpressed GLUT1, GLUT4, and chimeric transporter proteins.
Assessed trafficking alterations in fibroblasts and 3T3-L1 adipocytes following targeted mutations in N-terminal, C-terminal, and dileucine regulatory motifs.
Fibroblast trafficking conformed to a two-compartment model where slower GLUT4 exocytosis to the plasma membrane drives distribution differences relative to GLUT1.
Adipocyte trafficking required a three-compartment model incorporating flow from endosomes, with the C-terminus reducing exocytosis and mediating sequestration into insulin-responsive vesicles.
Mutation of the C-terminal dileucine motif significantly impaired GLUT4 endocytosis across both cell types without accounting for the baseline kinetic divergence between wild-type GLUT1 and GLUT4.

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Implication

Kinetic modeling reveals distinct trafficking mechanisms of GLUT1 and GLUT4 in fibroblasts and adipocytes, indicating critical structural motifs regulating glucose transporter distribution.

Cite This Study

Yeh et al. (1995) studied this question.

synapsesocial.com/papers/6a88188d3fe66a1452bbfd92 https://doi.org/10.1021/bi00047a018

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Implication

Kinetic modeling reveals distinct trafficking mechanisms of GLUT1 and GLUT4 in fibroblasts and adipocytes, indicating critical structural motifs regulating glucose transporter distribution.

Kinetic analysis of glucose transporter trafficking in fibroblasts and adipocytes

Key Points

Discussion

Implication

Cite This Study

Kinetic analysis of glucose transporter trafficking in fibroblasts and adipocytes

Key Points

Discussion

Implication

Cite This Study

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