AIMS: The purpose of this study was to determine the proficiency of supplements to enhance the recovery of Salmonella from heat-treated liquid egg albumen on solid agar media. METHODS AND RESULTS: Salmonella-inoculated albumen, heated at 53.3 degrees C for 4 min, was plated on 39 combinations of solid media with or without the addition of 12 supplements. Greater numbers of Salmonella (P < 0.05) recovered with the addition of 1.0 g l(-1) ferrous sulfate (FeSO(4)) than with any other supplements, except for 0.5 or 1.0 g l(-1) 3'3'-thiodipropionic acid (TDP), which recovered equivalent populations. Addition of 1.0 g l(-1) sodium pyruvate or 6.0 g l(-1) yeast extract plus 1.0 g l(-1) sodium pyruvate supported greater resuscitation than unsupplemented tryptic soy agar (TSA) or supplementing with 0.01 or 0.1 g l(-1) N-propyl gallate, 10 g l(-1) activated charcoal, 0.1 g l(-1) KMnO(4) or 50 mg l(-1) ethoxyquin. The remaining supplements supported recovery of equivalent numbers of Salmonella, which were fewer cells than recovered with 1.0 g l(-1) FeSO(4), yet greater populations than recovered with 50 mg l(-1) ethoxyquin. CONCLUSION: Supplementation of plating media with FeSO(4), TDP or sodium pyruvate enhanced recovery of sublethally injured Salmonella from albumen. SIGNIFICANCE AND IMPACT OF THE STUDY: Pasteurizing albumen impedes recovery of pathogens. These results suggest that the addition of supplements to plating media may assist resuscitation and colony development of heat-injured salmonellae.
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Gurtler et al. (2009) studied this question.
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