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What is the most sensitive assay for detecting serum antibody responses to the RIT 4237 rotavirus vaccine in infants and newborns?
What is the most sensitive assay for detecting serum antibody responses to the RIT 4237 rotavirus vaccine in infants and newborns?
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ELISA-IgM combined with IgG or NT is optimal for detecting rotavirus vaccine responses in older infants, whereas NT is most sensitive in newborns due to maternal antibodies.
Immunoglobulin class-specific determination by ELISA and a virus neutralization test (NT) were compared for the detection of the serum antibody responses after vaccination of infants with the RIT 4237 rotavirus vaccine (of bovine origin). A capture method was applied for specific IgM determination by ELISA because of its greater sensitivity and reproducibility over the conventional ELISA-IgM test. NT was improved through the use of an enzyme-labelled antibody for the detection of non-neutralized virus. In 6-12-month-old children, the most sensitive single test to detect an antibody response was the ELISA-IgM capture method, but the combination of ELISA-IgM and IgG tests or ELISA-IgM and NT detected the highest seroconversion rate of 79%. In newborn infants high levels of maternal antibody made the ELISA-IgG test unsuitable. ELISA-IgM gave a response rate of 31%, but NT with homologous virus was the most sensitive indicator of a serological response to the vaccine in this age group, yielding a 45% response rate.
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Delem et al. (1987) studied this question.
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