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June 1, 2000Journal of Biological ChemistryOpen Access

Sustained Endothelial Nitric-oxide Synthase Activation Requires Capacitative Ca2+ Entry

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Population

COS-7 cells transfected with chimera containing full-length eNOS cDNA and HA-tagged aequorin sequence and…

Comparison

Capacitative Ca2+ entry (CCE) vs Intracellular Ca2+ release or Ca2+ ionophore…

Design

Preclinical

Authors

SLSui LinKFKent A. FaganKLKai-Xun Li

Discussion

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Overview

Hypothesis-generating for endothelial eNOS regulation; leaves open in vivo relevance for vascular NO production.

Structured PICO

P
Population
COS-7 cells transfected with chimera containing full-length eNOS cDNA and HA-tagged aequorin sequence (EHA) and myristoylation-deficient EHA (MHA)
I
Intervention
Capacitative Ca2+ entry (CCE)
C
Comparator
Intracellular Ca2+ release or Ca2+ ionophore ionomycin
O
Outcome
eNOS activation and NO productionsurrogate

Capacitative Ca2+ influx is the principal stimulus for sustained activation of plasma membrane-associated eNOS in intact cells.

Cite This Study

Lin et al. (2000) studied this question.

synapsesocial.com/papers/6a8845ea054eefc4d776fe66https://doi.org/10.1074/jbc.275.24.17979
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Endothelial nitric oxide synthase. N-terminal myristoylation determines subcellular localization.1993 · 276 citations
  2. 2Calmodulin-dependent endothelium-derived relaxing factor/nitric oxide synthase activity is present in the particulate and cytosolic fractions of bovine aortic endothelial cells.1991 · 594 citations
  3. 3Phosphorylation and subcellular translocation of endothelial nitric oxide synthase.1993 · 325 citations
  4. 4Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform.1992 · 992 citations
  5. 5A new generation of Ca2+ indicators with greatly improved fluorescence properties.1985 · 21,790 citations