An Opportunity to distingush species of Saccharomyces Sensu stricto by electrophoretic separation of the larger Chromosomes

Intact chromosomal DNA of the type strains of Saccharomyces sensu stricto species and some of their synonyms and 38 Hungarian wine and 14 brewing yeast strains were separated by rotating field gel electrophoresis (RFE). The applied electrophoretic conditions enabled us to distinguish strains of S. bayanus, S. pastorianus and S. cerevisiae from each other, for strains of the three species showed clear differences in their electrophoretic patterns at heavy chromosomes (> 1300 kb).