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Spinach chloroplasts and their various fractions enriched in either photochemical reaction centre 1 (P700) or reaction centre 2 (P680) have been analyzed for pigment composition by high‐performance liquid chromatography (HPLC). The results clearly establish a stoichiometry of chlorophyll (Chl) a' /P700 = 2, where Chl a ' represents the C 10 epimer of Chl a . This is the first demonstration of the association of Chl a' with any functional site in vivo. It is further shown that a Chl a ' dimer prepared in vitro reproduces fairly well the redox and spectroscopic behaviour of P700. These findings provide strong evidence that a Chl a dimer constitutes P700.
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Watanabe et al. (1985) studied this question.
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