What question did this study set out to answer?

Develop and evaluate an integrated, label-free multimodal nonlinear optical microscope utilizing an erbium-doped femtosecond fiber laser for biological imaging.

December 13, 2024Microscopy Research and Technique

A Femtosecond Fiber Laser‐Based Compact Label‐Free Multimodality Nonlinear Optical Microscopy and Its Ex Vivo Bioimaging

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Authors

CJChen JiaNanjing University of Chinese Medicine QLQ.J. LiAnhui University ZZZhanqin ZhangFirst Affiliated Hospital of Xi'an Jiaotong University

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Implication

Ex vivo imaging study demonstrates simultaneous label-free multimodal nonlinear microscopy in plant and animal tissues, highlighting potential for deep bioimaging.

Key Points

Develop and evaluate an integrated, label-free multimodal nonlinear optical microscope utilizing an erbium-doped femtosecond fiber laser for biological imaging.
Engineered an integrated imaging system simultaneously generating second harmonic generation (SHG), third harmonic generation (THG), and three-photon excited fluorescence (3PEF) using an Er3+-doped femtosecond fiber laser.
Conducted ex vivo multimodality imaging across diverse plant tissues (potato starch, leaf chloroplasts, onion Hechtian strands) and mammalian skin structures.
Achieved simultaneous multimodal optical imaging with spatial resolutions of 1.67 μm for 3PEF, 1.5 μm for THG, and 2.67 μm for SHG modalities.
Successfully visualized label-free cellular and architectural components, including plant cell wall-to-membrane linkers, epidermal intercellular boundaries, hair follicles, and collagen.

Cite This Study

Jia et al. (2024) studied this question.

synapsesocial.com/papers/6a88527fd79ae02bd34e0641 https://doi.org/10.1002/jemt.24757

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Implication

Ex vivo imaging study demonstrates simultaneous label-free multimodal nonlinear microscopy in plant and animal tissues, highlighting potential for deep bioimaging.

A Femtosecond Fiber Laser‐Based Compact Label‐Free Multimodality Nonlinear Optical Microscopy and Its Ex Vivo Bioimaging

Discussion

Member takes

Implication

Key Points

Cite This Study

A Femtosecond Fiber Laser‐Based Compact Label‐Free Multimodality Nonlinear Optical Microscopy and Its Ex Vivo Bioimaging

Discussion

Member takes

Implication

Key Points

Cite This Study

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