Extract of lentil seeds in 80% acetone was prepared. Four fractions (I‐IV) were separated on a Sephadex LH‐20 column using methanol as the mobile phase. The antioxidant activity of each fraction was investigated in a β‐carotenelinoleate model system; UV spectra were recorded and the content of total phenolics determined. Results of β‐carotene‐linoleate model system indicated that antioxidant activities of separated fractions correlated with their content of total phenolic compounds and were in the order of IV>III>II>I. Fractions III and IV from lentil showed much stronger antioxidant activity than phenolics fractions separated from extracts of several different legumes using Sephadex LH‐20 column chromatography. Absorption maxima of UV spectra showed that flavonoids were the main phenolic compounds present in the separated fractions. Results of EI‐MS confirmed the presence of epicatechin in fraction III while condensed tannins were detected in fraction IV using TLC.
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Amarowicz et al. (2003) studied this question.
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