Key result
Neither cilazapril nor losartan affected tubular cell apoptosis in rats with obstructive nephropathy, while cilazapril significantly increased interstitial cell apoptosis (P=0.019).
Why the study?
Does cilazapril or losartan reduce tubular and interstitial cell apoptosis in rats with unilateral obstructive nephropathy?
Does cilazapril or losartan reduce tubular and interstitial cell apoptosis in rats with unilateral obstructive nephropathy?
p-value: p=0.019
In a rat model of obstructive nephropathy, ACE inhibition with cilazapril increased interstitial cell apoptosis, while neither ACEi nor ARB affected tubular cell apoptosis.
No clinical implications for ACEi/ARB use in obstructive nephropathy; leaves open differential interstitial apoptotic effects for translational study.
AIM: To investigate the effects of angiotensin-converting enzyme inhibitor (cilazapril) and angiotensin II type I receptor antagonist (losartan) on tubular and interstitial cell apoptosis and caspase-3 activity in rats with obstructive nephropathy after unilateral ureteral obstruction. METHODS: Rats with unilateral obstructive nephropathy and sham-operated rats were treated with cilazapril, losartan, or the vehicle (water). Tubular and interstitial cell apoptosis was detected morphologically on hematoxylin and eosin-stained renal specimens and by the terminal deoxynucleotidyl transferase-mediated nick end-labeling. Caspase-3 activity in whole-kidney tissue homogenates was measured colorimetrically. RESULTS: After unilateral ureter ligation, there was a significant increase in the number of apoptotic tubular and interstitial cells in the obstructed kidney (P=0.049 and P=0.036, respectively, vs sham-operated rats, 10 days after ligation). In rats with unilateral obstructive nephropathy, neither cilazapril nor losartan had an effect on tubular cell apoptosis. However, cilazapril caused a significant increase in the number of renal apoptotic interstitial cells (P=0.019). Caspase-3 activity was not significantly different in rats with unilateral obstructive nephropathy than in sham-operated rats. CONCLUSION: Rats with unilateral obstructive nephropathy had increased apoptosis of tubular and interstitial cells in comparison with sham-operated rats. Neither cilazapril nor losartan had an effect on tubular cell apoptosis, and cilazapril even increased interstitial cell apoptosis.
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Radović et al. (2008) studied Obstructive nephropathy. Cilazapril or losartan vs. Vehicle (water) was evaluated on Tubular and interstitial cell apoptosis and caspase-3 activity (p=0.019). Neither cilazapril nor losartan affected tubular cell apoptosis in rats with obstructive nephropathy, while cilazapril significantly increased interstitial cell apoptosis (P=0.019).
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