What question did this study set out to answer?

To evaluate and compare data-independent acquisition (DIA) and parallel reaction monitoring (PRM) mass spectrometry workflows for targeted quantification of low-abundance lysosomal proteins in complex biological samples.

January 26, 2021ProteomesOpen Access

Targeted Quantification of the Lysosomal Proteome in Complex Samples

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Authors

PMPeter MosenUniversity of Bonn ASAnne SannerUniversity of Bonn JSJasjot SinghMerck (Germany)

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Implication

Comparative proteomic study reveals distinct strengths of DIA and PRM across complex mouse samples, highlighting complementary methods for lysosome profiling.

Key Points

To evaluate and compare data-independent acquisition (DIA) and parallel reaction monitoring (PRM) mass spectrometry workflows for targeted quantification of low-abundance lysosomal proteins in complex biological samples.
Applied DIA and PRM mass spectrometry workflows to three distinct biological matrixes: a lysosome-enriched fraction, mouse embryonic fibroblast whole cell lysates, and mouse liver whole tissue lysates.
Assessed protein identification capacity and quantification performance across varying chromatographic gradient lengths.
Both DIA and PRM successfully identified and quantified lysosomal proteins across all sample types, yielding strongly correlated quantitative results.
DIA identified more lysosomal proteins on average in samples with lower complexity and when combined with longer chromatographic gradients.
PRM demonstrated superior identification and quantification performance in highly complex whole tissue samples analyzed using shorter chromatographic gradients.

Cite This Study

Mosen et al. (2021) studied this question.

synapsesocial.com/papers/6a885c2ec0c64b7dddeb46a2 https://doi.org/10.3390/proteomes9010004

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Implication

Comparative proteomic study reveals distinct strengths of DIA and PRM across complex mouse samples, highlighting complementary methods for lysosome profiling.

Targeted Quantification of the Lysosomal Proteome in Complex Samples

Discussion

Member takes

Implication

Key Points

Cite This Study

Targeted Quantification of the Lysosomal Proteome in Complex Samples

Discussion

Member takes

Implication

Key Points

Cite This Study

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