Answers are grounded in this paper and cite their sources.
ANO9 is identified as a cation channel activated by the cAMP/PKA pathway, potentially playing a role in intestinal function.
Anoctamins are membrane proteins that consist of 10 homologs. ANO1 and ANO2 are anion channels activated by intracellular calcium that meditate numerous physiological functions. ANO6 is a scramblase that redistributes phospholipids across the cell membrane. However, the others are not well characterized. We found ANO9/TMEM16J is a cation channel activated by a cAMP-dependent PKA. Intracellular cAMP activated robust currents in whole-cells expressing ANO9 and inhibited by PKA blockers. A cholera toxin and purified PKA also activated ANO9. The cAMP-induced ANO9 currents were permeable to cations. The mutation of a possible phosphorylation site at Ser245 elicited a block of the cAMP-dependent activation. High levels of Ano9 transcripts were found in intestines. Human intestinal SW480 cells showed cAMP-dependent currents. We conclude that ANO9 is a cation channel activated by the cAMP/PKA pathway and could play a role in intestine function.
No takes yet. Share an insight, caveat, or question.
Kim et al. (2017) studied this question.
Synapse has enriched 8 closely related papers on similar clinical questions. Consider them for comparative context: