Sensitivity Calibration of Multichannel Raman Spectrometers Using the Least-Squares-Fitted Fluorescence Spectrum of Quinine

A method of sensitivity calibration of multichannel Raman spectrometers is described which employs the fluorescence spectrum of quinine as an intensity standard. The reported absolute fluorescence spectrum of quinine was least-squares fitted with a polynomial so that it gave an interpolation of the spectrum in the “Raman scale.” The obtained least-squares-fitted fluorescence spectrum was cross-checked with the rotational Raman spectrum of D 2 . The fluorescence intensities calculated for every 100 cm −1 from the two Ar + laser lines are tabulated for convenience.