What question did this study set out to answer?

To identify the genetic alterations and molecular mechanisms driving multidrug resistance in spontaneous Bacillus subtilis mutants isolated under antibiotic selection.

February 1, 2003Canadian Journal of Microbiology

Multidrug resistant phenotype of Bacillus subtilis spontaneous mutants isolated in the presence of puromycin and lincomycin

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Authors

MMM. MurataKyorin University SOShinichi OhnoTohoku University MKMiyuki KumanoUniversity of Tsukuba

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Implication

Laboratory study reveals multidrug resistance in Bacillus subtilis mutants via lmrAB operon mutations, indicating that LmrA autogenously represses drug efflux pump LmrB.

Key Points

To identify the genetic alterations and molecular mechanisms driving multidrug resistance in spontaneous Bacillus subtilis mutants isolated under antibiotic selection.
Isolated spontaneous mutants by culturing Bacillus subtilis 168 in the presence of lethal concentrations of puromycin and lincomycin.
Profiled multidrug cross-resistance phenotypes and quantified lmrAB operon expression via Northern hybridization analysis.
Identified causal mutations by sequencing the regulatory and coding regions of the lmrAB locus.
Isolated mutants developed cross-resistance to multiple diverse antibiotic classes alongside a greater than 30-fold increase in lmrAB expression.
Resistance was caused by two distinct mutation classes: nucleotide substitutions in the region between the promoter and start codon of lmrA, and missense mutations within the LmrA coding sequence.
Findings established LmrB as a multidrug efflux transporter and LmrA as an autogenous transcriptional repressor of the lmrAB operon.

Cite This Study

Murata et al. (2003) studied this question.

synapsesocial.com/papers/6a8874f6c16cbaca05f4d615 https://doi.org/10.1139/w03-014

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Implication

Laboratory study reveals multidrug resistance in Bacillus subtilis mutants via lmrAB operon mutations, indicating that LmrA autogenously represses drug efflux pump LmrB.

Multidrug resistant phenotype of Bacillus subtilis spontaneous mutants isolated in the presence of puromycin and lincomycin

Discussion

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Implication

Key Points

Cite This Study

Multidrug resistant phenotype of Bacillus subtilis spontaneous mutants isolated in the presence of puromycin and lincomycin

Discussion

Member takes

Implication

Key Points

Cite This Study

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