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August 1, 1965Journal of Cellular and Comparative Physiology

The exchange of C14 glucose across the membrane of the human erythrocyte

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Authors

RMRichard C. MaweUniversity of California, San DiegoHHH. G. HemplingMedical University of South Carolina

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Implication

Laboratory study demonstrates faster transit of glucose-loaded carriers across human erythrocytes, indicating substrate-accelerated transport dynamics.

Key Points

  • To determine the kinetics of equilibrium and net efflux of radiolabeled glucose across human red blood cell membranes and assess carrier transport dynamics.
  • Developed an ultrafiltration sampling technique using millipore filter discs to measure isotope loss with flux half-times as short as 5 seconds.
  • Measured equilibrium efflux (equal glucose on both sides) and net efflux into a glucose-free medium across concentrations down to 0.03 M using paired blood samples.
  • Tracer exchange half-times decreased consistently as equilibrium glucose concentrations were reduced down to 0.03 M.
  • Direct comparison between equilibrium and maximal net fluxes revealed that the glucose-bound carrier transits the membrane more rapidly than the unbound carrier.

Cite This Study

Mawe et al. (1965) studied this question.

synapsesocial.com/papers/6a8875742c11edba2ecf29b6https://doi.org/10.1002/jcp.1030660110
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Also Consider

Synapse has enriched one closely related paper. Consider it for comparative context:

  1. 1EQUATIONS FOR TRACER EXPERIMENTS1949 · 148 citations