Recent progress in the elucidation of the biological role of nucleic acids has led to the postulation of a mechanism for the transfer of information from DNA1 to RNA.Reports from a few laboratories2 have indicated that a hybrid molecule containing DNA and RNA may be an intermediate in this process.This report is concerned with the isolation and characterization of a naturally occurring DNA- RNA complex from the fungus Neurospora crassa which has some of the properties required of such an intermediate.Materials and Methods.-Growth of Neurospora crassa strain 74A: Cultures were maintained on agar slants of minimal medium.3Freshly inoculated slants were incubated at 300C for 5 days and then stored at 3-50C.Experimental material was obtained by suspending the conidia from one agar slant in 10 ml of distilled water and adding this suspension to 1 liter of liquid minimal medium contained in a Fernbach flask.The flasks were then incubated at 300C with vigorous agitation on a rotary shaker.
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Schulman et al. (1962) studied this question.