Key Points
- To describe and characterize a filtration pressure method for measuring platelet aggregation directly in whole blood.
- Induced platelet aggregation in whole blood under standardized conditions using adenosine diphosphate (ADP).
- Measured filtration resistance and systematically evaluated the effects of ADP concentration, anticoagulant type and dose, mechanical agitation, post-venepuncture interval, incubation duration, platelet count, and haematocrit.
- Filtration pressure directly reflects the degree of platelet aggregation induced within whole blood.
- Pressure measurements are significantly influenced by procedural and physiological variables, including anticoagulant concentration, timing after blood draw, agitation, and haematocrit.
Structured PICO
PPopulationWhole blood samples
IInterventionPlatelet aggregate filtration pressure method
OOutcomeMeasurement of platelet aggregationsurrogate
Describes a novel method for measuring platelet aggregation in whole blood based on filtration characteristics.