Effect of varying enzyme concentration on the action of rennin on whole milk

Summary The kinetics of the release of glycopeptides by the action of rennin on bovine whole milk have been studied. The initial rate of release of glycopeptides was proportional to the concentration of rennin. Using the integrated form of the Michaelis–Menten equation, values have been obtained forKmandV. These values varied between milk samples from individual cows, and for a single milk sample the value forKmincreased with increasing rennin concentration. It is suggested that theKmvalue is partly dependent on the carbohydrate composition of individual κ-casein molecules.