During the rennet curdling of milk, the amount of nitrogen (NPN) which is not precipitated by 12% trichloroacetic acid (TCE) increases markedly. The course of this splitting reaction can be followed quantitatively. The NPN/time curves show clearly that a very specific reaction is involved which terminates before visible clotting occurs and which differs distinctly from the slow but general proteolytic breakdown effected by the enzyme.
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Alais et al. (1953) studied this question.