January 18, 2011Proceedings of the National Academy of SciencesOpen Access

A modest decrease in endothelial NOS in mice comparable to that associated with human NOS3 variants exacerbates diabetic nephropathy

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Why the study?

Does a modest genetic decrease in eNOS expression exacerbate diabetic nephropathy in a mouse model?

Population

F1 progeny of a cross between heterozygous 129S6/SvEvTac eNOS+/- inbred females and heterozygous C57BL/6J…

Comparison

Genetic reduction or absence of endothelial… vs Wild-type eNOS mice with or without the Akita…

Design

Preclinical

Follow-up

7 months

Authors

CWChih-Hong WangTulane University FLFeng LiChangchun University of Science and Technology SHSylvia HillerUniversity of North Carolina at Chapel Hill

Discussion

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Implication

Suggests eNOS variants may worsen diabetic nephropathy; leaves open relevance of human NOS3 polymorphisms to clinical outcomes.

Structured PICO

Does a modest genetic decrease in eNOS expression exacerbate diabetic nephropathy in a mouse model?

Population

F1 progeny (eNOS+/+, eNOS+/-, or eNOS-/- with or without diabetes) of a cross between heterozygous 129S6/SvEvTac eNOS+/- inbred females and heterozygous C57BL/6J eNOS+/- inbred males carrying the dominant Akita diabetogenic mutation Ins2(C96Y/+).

Intervention

Genetic reduction or absence of endothelial nitric oxide synthase (eNOS+/- or eNOS-/-)

Comparator

Wild-type eNOS (eNOS+/+) mice with or without the Akita diabetogenic mutation

Outcome

Severity of diabetic nephropathy (urinary albumin excretion, mesangial expansion, glomerulosclerosis, mesangiolysis, and glomerular filtration rate)surrogate

A modest decrease in eNOS expression, comparable to human NOS3 variants, exacerbates diabetic nephropathy independently of its effects on blood pressure.

Cite This Study

Wang et al. (2011) studied this question.

synapsesocial.com/papers/6a8907fc30fc94fc4b130e07 https://doi.org/10.1073/pnas.1018766108

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A modest decrease in endothelial NOS in mice comparable to that associated with human NOS3 variants exacerbates diabetic nephropathy

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A modest decrease in endothelial NOS in mice comparable to that associated with human NOS3 variants exacerbates diabetic nephropathy

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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