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Unstimulated and phytohemagglutinin-stimulated lymphocytes from nine human subjects were suspended in Cetrimide solution before and after tissue culture. Their nuclear volumes were measured serially with a particle counter—400 channel analyzer device. Variations of the volume distribution curves correlated well with the morphological changes associated with blastoid transformation. This method permits rapid and objective assay of lymphocyte transformation.
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Muniz et al. (1970) studied this question.
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