What question did this study set out to answer?

To develop and evaluate a rapid histochemical assay for detecting localized hydrogen peroxide generation in plant tissues undergoing lignification.

November 1, 1993The Plant JournalOpen Access

Hydrogen peroxide and lignification

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Authors

POPaul OlsonWashington University in St. Louis JVJ. E. VarnerWashington University in St. Louis

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Implication

Experimental study demonstrates localized hydrogen peroxide production in lignifying plant cells using an iodide-starch assay, indicating an active role of reactive oxygen in cell wall formation.

Key Points

To develop and evaluate a rapid histochemical assay for detecting localized hydrogen peroxide generation in plant tissues undergoing lignification.
Applied a test solution of 50 mM potassium iodide in a 4% (w/v) potato starch suspension directly to cut surfaces of plant tissues.
Tested reaction specificity by observing color change under anaerobic conditions and in the presence of catalase.
Hydrogen peroxide oxidized iodide to iodine, forming a blue-purple starch complex in lignifying tracheary elements, phloem fibers, and select epidermal cells.
Rapid hydrogen peroxide production was detected in crushed cells, while signal formation was completely absent under anaerobic conditions and with catalase treatment.

Cite This Study

Olson et al. (1993) studied this question.

synapsesocial.com/papers/6a8923d258d99da5144d37cc https://doi.org/10.1046/j.1365-313x.1993.04050887.x

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Implication

Experimental study demonstrates localized hydrogen peroxide production in lignifying plant cells using an iodide-starch assay, indicating an active role of reactive oxygen in cell wall formation.

Hydrogen peroxide and lignification

Discussion

Member takes

Implication

Key Points

Cite This Study

Hydrogen peroxide and lignification

Discussion

Member takes

Implication

Key Points

Cite This Study

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