October 1, 1992Journal of Clinical InvestigationOpen Access

Microfilament-dependent activation of Na+/K+/2Cl- cotransport by cAMP in intestinal epithelial monolayers.

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Population

Human intestinal cell line T84 monolayers

Comparison

Phalloidin loading followed by forskolin… vs Control monolayers stimulated with forskolin

Design

Preclinical

Authors

JMJeffrey B. MatthewsWisdom Health (United States)CAC.S. AwtreyBeth Israel Deaconess Medical Center JMJames MadaraAmerican Medical Association

Discussion

Member takes

Implication

Cytoskeletal dependence of cAMP-stimulated NKCC in epithelia is hypothesis-generating; leaves open any clinical relevance to secretory disorders.

Structured PICO

Population

Human intestinal cell line T84 monolayers

Intervention

Phalloidin loading followed by forskolin stimulation

Comparator

Control monolayers (without phalloidin loading) stimulated with forskolin

Outcome

Activity of individual membrane-associated transport proteins (Cl- and K+ conductances, Na+/K+-ATPase pump activity, Na+/K+/2Cl- cotransport)surrogate

The cAMP-induced activation of the Na+/K+/2Cl- cotransporter is microfilament-dependent, suggesting it is functionally linked to the cytoskeleton in intestinal epithelial cells.

Cite This Study

Matthews et al. (1992) studied this question.

synapsesocial.com/papers/6a892effab71709247a2b9f2 https://doi.org/10.1172/jci116030

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Implication

Cytoskeletal dependence of cAMP-stimulated NKCC in epithelia is hypothesis-generating; leaves open any clinical relevance to secretory disorders.

Microfilament-dependent activation of Na+/K+/2Cl- cotransport by cAMP in intestinal epithelial monolayers.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Microfilament-dependent activation of Na+/K+/2Cl- cotransport by cAMP in intestinal epithelial monolayers.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider