The susceptibility of in vivo-grownPasteurella haemolyticato ovine defence mechanisms in vitro

Pasteurella haemolytica organisms grown in vivo were examined for their susceptibility to ovine immune mechanisms in vitro. Compared with in vitro grown organisms they were less susceptible to opsonophagocytosis and, in contrast, susceptible to complement-dependent killing in the absence of exogenous antibody. These differences were not associated with phenotypic changes in the surface of the bacterial cell. However, overproduction and de novo synthesis of proteins was observed in in vivo grown organisms. Also, bound host-immunoglobulin was observed on in vivo grown organisms and a role for this in modifying the interaction with immune mechanisms is discussed.