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Liquid-column chromatography (LCC), which was the earliest chromatographic technique to be applied to steroids, has become the most recent and most promising addition to the armamentarium of the steroid analyst in the form of high-performance liquid chromatography (HPLC). Conventional column chromatography (CC) has survived the competition with planar and gas chromatography (GC) in the steroid field, because it offers greater capacity and a greater variety of experimental conditions. Both of these advantages are important in the analysis of steroids, because in nature steroids occur as complex mixtures of disparate concentrations and polarites. By virtue of their relatively large capacity, columns can be loaded with enough crude mixture to allow the detection of trace amounts of certain components. Steroids of diverse polarities can be fractionated by a wide range of sorbents and eluents, e.g., by gradient elution.
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Heftmanń et al. (1982) studied this question.
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