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October 1, 1990Journal of VirologyOpen Access

Analysis of mutations in the integration function of Moloney murine leukemia virus: effects on DNA binding and cutting

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Population

Moloney murine leukemia virus integration (IN) protein and Escherichia coli plasmid expressing the IN protein

Comparison

Six linker insertion mutations and two… vs Parent/wild-type IN protein

Design

Preclinical

Authors

MRMonica J. RothJohnson UniversityPSPamela L. SchwartzbergNational Institutes of HealthNTNaoko TaneseNew York University

Discussion

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Implication

May guide integrase-targeted antivirals; leaves open translation from this murine model to human applications.

Structured PICO

P
Population
Moloney murine leukemia virus integration (IN) protein and Escherichia coli plasmid expressing the IN protein
I
Intervention
Six linker insertion mutations and two single-base substitutions within the region encoding the IN protein
C
Comparator
Parent/wild-type IN protein
O
Outcome
DNA-binding activity and formation of 3' recessed termini preceding integration

Mutations in the IN protein of Moloney murine leukemia virus block the formation of 3' recessed termini, preventing viral integration.

Cite This Study

Roth et al. (1990) studied this question.

synapsesocial.com/papers/6a893582abb0ef83c5c9765fhttps://doi.org/10.1128/jvi.64.10.4709-4717.1990
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Analysis of mutant Moloney murine leukemia viruses containing linker insertion mutations in the 3' region of pol1988 · 33 citations
  2. 2The avian retroviral integration protein cleaves the terminal sequences of linear viral DNA at the in vivo sites of integration1989 · 313 citations
  3. 3The detection of DNA-binding proteins by protein blotting1980 · 735 citations
  4. 4Splice acceptor site for the env message of Moloney murine leukemia virus1987 · 30 citations
  5. 5Integration of mini-retroviral DNA: a cell-free reaction for biochemical analysis of retroviral integration.1989 · 111 citations