What question did this study set out to answer?

To develop and evaluate a polyvalent immunofluorescent intracellular antigen assay for rapid screening of antibodies to Lassa, Marburg, and Ebola viruses.

November 1, 1981Journal of Clinical MicrobiologyOpen Access

Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys

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Authors

KJKarl M. JohnsonUniversity of New Mexico LELarry ElliottPeace Corps DHDominique HeymannSmithsonian Institution

Discussion

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Implication

Diagnostic assay study demonstrates equivalent antibody detection sensitivity across filoviruses and arenaviruses, suggesting efficient multiplexed screening for human serosurveys.

Key Points

To develop and evaluate a polyvalent immunofluorescent intracellular antigen assay for rapid screening of antibodies to Lassa, Marburg, and Ebola viruses.
Mixed Vero cells individually infected with Lassa, Marburg, or Ebola viruses onto Teflon-templated microscopy slides.
Evaluated the polyvalent cell mixtures against standard monovalent antigens for detecting and quantitating viral antibodies.
The polyvalent antigen preparation demonstrated diagnostic sensitivity equal to monovalent antigen preparations for detecting antibodies to all three hazardous viral pathogens, though specific quantitative values were not reported.
The pooled-cell microscopy method permitted simultaneous screening and antibody quantitation within a single slide preparation.

Cite This Study

Johnson et al. (1981) studied this question.

synapsesocial.com/papers/6a8937831fe1bad943eb1673 https://doi.org/10.1128/jcm.14.5.527-529.1981

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Implication

Diagnostic assay study demonstrates equivalent antibody detection sensitivity across filoviruses and arenaviruses, suggesting efficient multiplexed screening for human serosurveys.

Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys

Discussion

Member takes

Implication

Key Points

Cite This Study

Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys

Discussion

Member takes

Implication

Key Points

Cite This Study

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