A novel fluorescent probe, quinoline α,β-unsaturated diacid (QMA), as compared to its ester QME, was constructed and can selectively detect thiols in acidic solutions (pH < 7) via a H-bond activated Michael addition. Furthermore, labeling of lysosomes and cell imaging were achieved by the detection of biothiols in different domains of live cells.
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Song et al. (2012) studied this question.
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